Cytofectin
WebFeb 17, 2006 · Abstract. The synthesis and transfection potential of a novel cationic cholesterol cytofectin with a dimethylamino head group and a long 12 atom, 15 Å … WebAug 1, 1996 · To prepare the cytofectin/ON complexes for transfection in one well of a 6-well tissue culture plate, the ON was first diluted into 100 µl pre-warmed Opti-MEM (Gibco BRL) in polystyrene plastic. Similarly, the cytofectin was diluted in another container ( 5 ).
Cytofectin
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Webwe introduced Cytofectin GSV, a transfection reagent, designed at and licensed by Gilead, to be used to transport antisense oligonucleotides into cells even in the presence of … WebProvided is a collection of cytofectins (cytofectins are defined as chemical species that are cationic transfection amphiphiles) or cationic lipids that bind and transport polynucleotides,...
WebAug 30, 2002 · Using Cytofectin GSV to deliver both inhibitors, the siRNAs appear to be quantitatively more efficient and its effect is lasting for a longer time in cell culture. In mice, we observed an activity of siRNAs but not of antisense oligonucleotides. WebJul 25, 2024 · Cationic liposomes are potential vectors for gene therapy applications. In previous work, our first generation cationic liposome system, formulated from cytofectin 3β-[N-(N ′,N ′-dimethylaminoethyl)carbamoyl]cholesterol (DC-Chol) and the neutral phospholipid dioleoyl-L-α-phosphatidylethanolamine (DOPE), was shown to transfect the lungs of …
WebA cytofectin according to the present invention can include a molecular species having a biological cell targeting activity as a terminal group. Within this class are cytofectins comprising cell receptor-specific molecules. Typically, the receptor-specific peptides or amino acids are linked as amides. Examples of preferred peptides which could ... Webproved cytofectin, termed GS 2888, which overcomes many of the shortcomings of Lipofectin for delivering ODNs and plasmids to cells. The GS 2888 cytofectin efficiently transfects ODNs and plasmids into many cell types in the presence or absence of serum in the medium, uses a 4- to 10-fold lower concentration of the agent as compared to ...
WebAug 4, 1997 · This is the first report on the biodistribution of the cytofectin component of a pDNA–cationic lipid complex for which the distribution of the plasmid component has …
WebApr 1, 2001 · Cytofectin formulations have also been optimized for vaccination applications. Vaxfectin, a new cytofectin formulation, has recently shown significant enhancement of humoral immune responses against several antigens compared to naked pDNA ( 19 ). sharita tanner obituaryWebCytofectin proved more efficient than Superfect in the delivery of chimeric oligonucleotide into mouse skin (data not shown). In addition, Cytofectin penetrated the stratum corneum, which provides ... popsicle christmas tree craftWebNational Center for Biotechnology Information popsicle christmas ornamentsWebThe transfection activity of cytofectin formulations depends greatly on the cationic lipid chemical species, on the presence of particular neutral co-lipids in the formulation, on the … popsicle cloth diaperWebFeb 5, 1999 · A 1/3 (w/w) ratio of DNA/liposome (1/1 nucleotide/ cytofectin mol ratio) was determined to be optimal for transfection. In addition, we found that the transfection complexes were able to penetrate ... popsicle clickerWebAlthough optimal for the transfection of antisense oligonucleotides, Cytofectin GSV has also been found to be suitable for plasmid transfection. In December 1998, Gilead's antisense technology, including the patents covering the C-5 Propynes and Cytofectin GSV, was purchased by Isis Pharmaceuticals, Inc. sharita thompsonWebAS-ODN with cytofectin compared with the corresponding S-ODN with cytofectin (530 190 lm, n = 3, >87 PTs per replicate; Figure S2). The pollen germination rate was not significantly reduced by the AS-ODN with cytofectin (59 5%, n = 3, >310 PTs per experiment) compared with the corresponding S-ODN with cytofectin (64 10%, n = 3, sharita thomas unc