Flp in cho
WebFlp-In™ Cell Lines are designed for rapid generation of stable cell lines that express a protein of interest from a Flp-In™ expression vector. These cells contain Invitrogen Flp … WebMar 5, 2024 · We confirmed that SLC20A2 variants caused the loss of function of the Pi transport activity in both Flp-In CHO cells and disease-specific iPSCs. This is the first report to show an in vitro model of iPSCs in IBGC with patient-identified SLC20A2 variants. These useful tools will help in elucidating IBGC pathogenesis and can be used for screening ...
Flp in cho
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WebIntroduction The Flp-In™System allows integration and expression of your gene of interest in mammalian cells at a specific genomic location. The Flp-In™System involves … WebJul 17, 2024 · (C) Commercially available Flp-in tm CHO cells were used as a positive control comparator following transfection with pcDNA5/FRT plasmid carrying the AQP3-GFP gene. Scale bar = 30 μm. (D) The full …
WebFeb 22, 2006 · The Flp-In CHO cells contain an Flp recombination target (FRT) site in their genome and allow for the integration of the pcDNA5/FRT expression vector at the FRT site via Flp recombinase-mediated DNA recombination ( O'Gorman et al., 1991 ). WebThe Flp-In-CHO Cell Line was created by transfecting CHO cells with pFRT/ lac Zeo2 and selecting for Zeocin™-resistant clones Flp-In-CHO cell lines work well with Flp-In vectors …
WebA single gene copy of shRNA-2 also decreased the translational level of DHFR by 80% in Flp-In CHO cells. shRNA-2 was then incorporated into a plasmid vector expressing human erythropoietin (EPO) and an exogenous DHFR to develop … WebFeb 11, 2024 · Results: A range of sialyltransferase from mammals, birds and fish were stably expressed in CHO Flp-In cells. The stable cell lines expressing ST6Gal1 modify the glycans on the surface of the CHO cells as detected by fluorescently labelled lectin microscopy. The catalytic domains, as isolated by Ni Sepharose from culture media, …
WebTransferring CHO Cells From Monolayer to Suspension Culture Decant medium from a T-25 flask and wash monolayer with 3 ml (5 ml for a T-75 flask) of Calcium and Magnesium-free D-PBS, Cat. No. 14190. Culture should be 50 to 80% confluent to ensure that the cells are actively dividing. Decant PBS.
WebThe Flp-In™ cell lines stably express the lacZ-Zeocin™ fusion gene and are designed for use with the Flp-In™ System (Cat. nos. K6010-01 and K6010-02). Each cell line contains … greenleaf rent a car santa anaWebOct 1, 2024 · Objective To construct a double transfected Flp-InTM CHO cell line stably expressing both cytochrome P450 family 2 subfamily A member 13(CYP2A13) and … fly goairgreenleaf researchWebSep 19, 2024 · Thus, FLP-20 peptides function as an afferent arousal signal that conveys mechanosensory information to central neurons that modulate arousal and other … green leaf residential care maineWebObjective To construct a double transfected Flp-In TM CHO cell line stably expressing both cytochrome P450 family 2 subfamily A member 13(CYP2A13) and multidrug resistance … flyg miami new yorkWebDec 1, 2024 · Flp-In-CHO cells (Life Technologies) were maintained in F12 medium (Gibco) supplemented with 10% heat-inactivated fetal bovine serum (FBS; Gibco) at 37 °C in a humidified incubator containing 5% CO 2. The cells were transfected with 1 μg plasmid (pDF-λ1:pOG44 was 1:9) using Lipo 2000 (Life Technologies) according to the … greenleaf residential estateWebJun 21, 2024 · Among different recombinases, the Flp/FRT system has been extensively applied in CHO cells to express monoclonal antibodies 35 and recombinant proteins 34, 36 probably due to its high... flygo clothes