Web2. Aspirate the medium from the stock culture and rinse with 3ml Versene. 3. Add 1ml 0.05% trypsin-0.02% EDTA. 4. Incubate the flask at 37C until cells round up (1-5 minutes) 5. Add 5ml of growth medium to the flask and dislodge any undetached cells by gently pipetting the medium against the bottom surface of the flask. 6. http://dept.harpercollege.edu/chemistry/chm/100/dgodambe/thedisk/labtech/piptech.htm
The Use of Volumetric Pipets with NIST Handbook 133 Checking …
WebWhen aspirating liquid, hold the pipette vertically and pull the pipette straight out from the center of the reservoir. This technique is especially important when pipetting small volumes (less than 50µL). Holding the … Web9 jan. 2024 · Wash with hot soapy water, rinse thoroughly with tap water, then rinse three to four times with deionized water. Be sure the final rinses flow off of the glass. Burets need … checkplace preroutedrc
CHM138 LAB Report 1 - LABORATORY REPORT CHM138 (BASIC
Make sure all parts of your pipettes are completely dry before reassembling. Lightly lubricate the piston with silicone grease (usually provided with your pipette) and reassemble the pipette. And don’t forget the O-ring! Check the calibration of your pipettebefore using it again. Cleaning pipettes is just one … Meer weergeven Pipettes should be given a quick clean every day. To clean your pipette daily, wipe the outside surfaces with 70% ethanol. Pipettes should also have a deeper clean, performed at least every three months. … Meer weergeven No matter how careful you are, dirt and contaminants can get inside your pipette, where your daily clean won’t reach. Therefore it is wise to deep clean your pipette … Meer weergeven If your pipette has become contaminated (e.g., because you accidentally over-aspirated your sample), you will need to decontaminate it. The procedure for decontaminating your pipette depends on the … Meer weergeven WebFailure to pre-rinse Dispensing liquid from a pipette leaves a coating of the liquid on the tip, making the expelled volume slightly less than it should be. Pre-rinsing a new tip at least twice with the liquid about to be used will … Web12 apr. 2024 · 1. Introduction. Air pollution is a major global health concern. In 2024, The World Health Organization (WHO) estimated that 90% of the global population lived in areas which fail to meet their guideline concentration for ambient fine particulate matter (PM 2.5), an annual average of 10 µg m −3 [].In September 2024, this guideline value was revised … check pixels per inch